16 Coolest Western Blots in 2020

 

Western Blot Loading Control Antibody Pack 5 Vials #ad

Western Blot Loading Control Antibody Pack, 5 Vials

Western Blot Loading Control Antibody Pack, 5 Vials #ad - Size 5 vialshost rabbitconjugate unconjugatedimmunogen beta actin 50 ug wb h, m, r purified, beta tubulin50 ug wb bo, ch, d, h, m, mk, r, xe purified, gapdh 50 ug wb ca, chp, dros, h, m, mq, pi, r purified, histone h2b 50 ug wb ch, d, dros, h, m, ma, r, zf purified, anti-rabbit ig hrp secondary 50 ul wb ra purified, bo bovine cacat chchicken chpchimpanzee ddog drosdrosophila hhuman mmouse mamammals mkmonkey mqmacaque rrat xexenopus zfzebrafishapplications western blot.

 

Square - GeneMate Western Blot Boxes Blotting Containers #ad

Square – GeneMate Western Blot Boxes, Blotting Containers

Square – GeneMate Western Blot Boxes, Blotting Containers #ad - Ideal for halves, wholes, strips, or minis reduce antibody usage easy-to-open hinged lid reduces evaporation features 90° angle corners polystyrene.

 

Stellar Scientific - Western blot boxes 6 x 4 x 1 14in 152 x 102 x 32cm 5PK #ad

Stellar Scientific – Western blot boxes, 6 x 4 x 1 1/4in. 15.2 x 10.2 x 3.2cm, 5/PK

Stellar Scientific – Western blot boxes, 6 x 4 x 1 1/4in. 15.2 x 10.2 x 3.2cm, 5/PK #ad - Sturdy polystyrene boxes come in the most popular sizes.   Packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black.

 

Square - GeneMate Western Blot Boxes Blotting Containers #ad

Square – GeneMate Western Blot Boxes, Blotting Containers

Square – GeneMate Western Blot Boxes, Blotting Containers #ad - Ideal for halves, wholes, strips, or minis reduce antibody usage easy-to-open hinged lid reduces evaporation features 90° angle corners polystyrene.

 

Stellar Scientific - Western blot boxes 3 12 x 2 916 x 1in 89 x 65 x 25cm BLACK 5PK #ad

Stellar Scientific – Western blot boxes, 3 1/2 x 2 9/16 x 1in. 8.9 x 6.5 x 2.5cm, BLACK, 5/PK

Stellar Scientific – Western blot boxes, 3 1/2 x 2 9/16 x 1in. 8.9 x 6.5 x 2.5cm, BLACK, 5/PK #ad -   Packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black. Sturdy polystyrene boxes come in the most popular sizes.

 

Stellar Scientific - Western blot boxes 4 916 x 4 916 x 1 14in 116 x 116 x 32cm 5PK #ad

Stellar Scientific – Western blot boxes, 4 9/16 x 4 9/16 x 1 1/4in. 11.6 x 11.6 x 3.2cm, 5/PK

Stellar Scientific – Western blot boxes, 4 9/16 x 4 9/16 x 1 1/4in. 11.6 x 11.6 x 3.2cm, 5/PK #ad - Sturdy polystyrene boxes come in the most popular sizes.   Packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black.

 

Multibox 6-Compartment - GeneMate Western Blot Boxes Blotting Containers #ad

Multibox, 6-Compartment – GeneMate Western Blot Boxes, Blotting Containers

Multibox, 6-Compartment – GeneMate Western Blot Boxes, Blotting Containers #ad - Ideal for halves, wholes, strips, or minis reduce antibody usage easy-to-open hinged lid reduces evaporation features 90° angle corners polystyrene.

 

Western Blot ECL Kit with Enhancer Anti-Chicken BioAssay™ W1019-80A-30 Blots #ad

Western Blot ECL Kit with Enhancer, Anti-Chicken, BioAssay™, W1019-80A-30 Blots

Western Blot ECL Kit with Enhancer, Anti-Chicken, BioAssay™, W1019-80A-30 Blots #ad - One ng of purified rabbit muscle gapdh was serially diluted to 1 attogram and electrophoresis was performed. When testing by visible s. Note components in this kit are free of precipitate. The gel was transferred to a pvdf membrane, and then cut in the middle. Ultra-sensitive ecl western blot system that detects attogram (10e18g) or yoctomole (10e24m) protein levels. The right section was treated with enhancer, and the left was untreated. A film exposed for two minutes is shown above. Two convenient and affordable sizes 15 mini blots (1200cm2) and 30 mini blots (2500cm2). Fold enhancement varied from a few to a 1000-fold depending on the characteristics of antibodies and antigen used. Kit components w1019-80a1 enhancer, 1x50ml or 1x100ml w1019-80a12 antibody binding buffer, 20x, 1x60ml or 1x120ml w1019-80a3 blocking agent, 1x20g or 1x40g w1019-80a4 hrp conjugated secondary antibody (anti-chicken), 1x50ul or 1x100ul w1019-80a5 luminescence substrate solution a, 1x25ml or1x100ml w1019-80a6 luminescence substrate solution b, 1x25ml or 1x50ml storage and stability may be stored at 4°c. 10-e11g of antigen without treatment and 10e14g of gapdh with treatment gave similar signal strengths, indicating a 1000-fold enhancement. Stable for 6 months after receipt. Quality control duplicated western blots containing 10fg and 10pg of antigen are detected with anti-gapdh antibody. Immunoblotting was done using a primary antibody and hrp-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. Antigen at 10fg levels was detected only on the blot treated with enhancer, with similar intensity as antigen at 10pg level on the untreated blot. The enhancer utilizes a proprietary technology that increases antigen and antibody affinity and accessibility which can work with different antigens and antibodies, such as structural proteins (actin), enzymes (gapdh) or membrane proteins (egfr), increasing western blot signals dramatically.

 

Stellar Scientific - Western blot boxes 2 78 x 2 x 1 14in 73 x 51 x 32cm 5PK #ad

Stellar Scientific – Western blot boxes, 2 7/8 x 2 x 1 1/4in. 7.3 x 5.1 x 3.2cm 5/PK

Stellar Scientific – Western blot boxes, 2 7/8 x 2 x 1 1/4in. 7.3 x 5.1 x 3.2cm 5/PK #ad - Sturdy polystyrene boxes come in the most popular sizes.   Packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black.

 

Stellar Scientific - Western blot boxes 4 38 x 2 116 x 1 78in 111x 52 x 47cm 5PK #ad

Stellar Scientific – Western blot boxes, 4 3/8 x 2 1/16 x 1 7/8in. 11.1x 5.2 x 4.7cm, 5/PK

Stellar Scientific – Western blot boxes, 4 3/8 x 2 1/16 x 1 7/8in. 11.1x 5.2 x 4.7cm, 5/PK #ad - Sturdy polystyrene boxes come in the most popular sizes.   packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black.

 

Large - GeneMate Western Blot Boxes Blotting Containers #ad

Large – GeneMate Western Blot Boxes, Blotting Containers

Large – GeneMate Western Blot Boxes, Blotting Containers #ad - Ideal for halves, wholes, strips, or minis reduce antibody usage easy-to-open hinged lid reduces evaporation features 90° angle corners polystyrene.

 

Stellar Scientific - Western blot boxes 6 x 4 x 2in 152 x 102 x 51cm 5PK #ad

Stellar Scientific – Western blot boxes, 6 x 4 x 2in. 15.2 x 10.2 x 5.1cm, 5/PK

Stellar Scientific – Western blot boxes, 6 x 4 x 2in. 15.2 x 10.2 x 5.1cm, 5/PK #ad -   packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black. Sturdy polystyrene boxes come in the most popular sizes.

 

Stellar Scientific - Western blot boxes 3 12 x 2 916 x 1in 89 x 65 x 25cm 5PK #ad

Stellar Scientific – Western blot boxes, 3 1/2 x 2 9/16 x 1in. 8.9 x 6.5 x 2.5cm 5/PK

Stellar Scientific – Western blot boxes, 3 1/2 x 2 9/16 x 1in. 8.9 x 6.5 x 2.5cm 5/PK #ad - Sturdy polystyrene boxes come in the most popular sizes.   Packaged in packs of five boxes snug fitting lid prevents evaporation of precious reagent two sizes are available in both clear and black.

 

Med Rectangle - GeneMate Western Blot Boxes Blotting Containers #ad

Med. Rectangle – GeneMate Western Blot Boxes, Blotting Containers

Med. Rectangle – GeneMate Western Blot Boxes, Blotting Containers #ad - Ideal for halves, wholes, strips, or minis reduce antibody usage easy-to-open hinged lid reduces evaporation features 90° angle corners polystyrene.

 

Commassie Blue Staining Destaing Solution Western Blot Related Reagent #ad

Commassie Blue Staining Destaing Solution (Western Blot Related Reagent)

Commassie Blue Staining Destaing Solution (Western Blot Related Reagent) #ad - Remove the distilled water, and cover the gel with of the gel-washing solution and a few volume of coomassie staining solution. Continue staining until the protein bands are seen without background staining of the gel. Description usually after electrophoresis, researchers should evaluate the result through staining and destaining, and detect the protein which was disassembled into different bands from the gel. No band is seen maybe sample is not enough. 2. 3. This solution is a coomassie blue g-250 dye-based, can be widely used for rapid staining of protein electrophoresis gel of sds-page or non-denaturing page, as well as in the residual protein detection of page gel after by western transferring. Keep pouring distaining solution on gel and shake until its color turns to more light and background is clear. Shake while washing. 4. After electrophoresis, place gel in the distilled water. Appnotes faq 1. Shake slowly 3 times, 5 min each. 2. High background maybe impurity is not removed completely. Try to add two bsa lanes with different volumes when electrophoresis as a positive control. Usually it takes 5-6 hours or overnight at 4 degree c. All solutions should better just immerging gel. By this means, the working range for protein electrophoresis bands can be as low as 10ng. Try to wash longer or wash more times. This production is acidic and its corrosion requires a necessary protection when use it. Besides, researchers also could electroblotting the gel for different research purpose. At the end of this time, remove the solution by aspiration. Protocol 1. The purpose of this step is to gently remove the interference of sds and salt. Retrieve the staining solution, wash quickly with water, then add destaining solution.

 

MTCBio - MiniGel Western Blot Box for InvitrogenNovex mini-gels Black 10CS #ad

MTCBio – MiniGel Western Blot Box for Invitrogen/Novex mini-gels, Black, 10/CS

MTCBio – MiniGel Western Blot Box for Invitrogen/Novex mini-gels, Black, 10/CS #ad - The latest addition to our line of western blot boxes, this light-safe, black-opaque box is perfect for fluorescent stains and all your mini-gel work.   Designed for the novex system, but also compatible with other leading mini-gels.   Each box features a smooth, flat surface to allow for complete gel coverage while sparing precious reagents. Dimensions are 85 x 85 x 30mm  tight-fitting, easy to pull off lid  packaged in cases of 10 boxes   novex is a registered trademark of thermofisher.





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